Metabolite Processing Essay Example | Topics and Well Written Essays - 2250 words

Metabolite Processing - Essay Example According to the paper the scientists have identified many metabolites related to cancer. Some of the most important molecules are phosphocholine, glycine, glutamate, aspartate and essential aminoacids. Metabolic reprogramming alters the nutrient intake and excretion. The study of the metabolic pathways involved in the transformed cells is required for the study and treatment of cancer. There are about 60 human cancer cell lines producing nine types of tumors. The metabolic pathways present in these cell lines must be studied for the treatment of cancer in the humans. The quantitative analysis of the metabolites in the given cancer cell line are done using different biotechnological techniques. Some of the analysis methods like gas chromatography time- of – flight mass spectrophotometry, orthogonal partial least squares discriminate analysis, and acquity ultra performance liquid chromatography – quadrupole time – of – flight mass spectrphotometry, Liquid c hromatography – tandem mass spectrometry and micro array data analysis. These methods are used for the analysis of the spent medium and the base line medium of the tissue. From this study it is clear that   219 metabolites were analyzed from the cancer cell lines. Out of them, 111 metabolites were found in the spent medium and baseline medium. The major metabolites that are utilized from the medium are analyzed and the data were compared with the existing microarray pooled data. The studies have found that the rapid proliferation requires rapid utilization and synthesis of the metabolites in the medium.   Glycine is a non-essential amino acid and they are not synthesized directly in the body. Glycine is produced by the conversion of L-serine into glycine by the mitochondrial serine hydroxymethyltransferase. The analysis of the glycine concentration for the rapid cell proliferation and the effect of glycine in cancer cell proliferation were analysed in this technique. The Gene silencing technique was used for the analysis of the enzyme mitochondrial serine hydroxymethyltransferase. It was observed that the glycine consumption was very less in the absence of this enzyme and the cell proliferation was very less in the glycine depriving medium. When glycine was supplied externally, the cell showed rapid cell